Herald R Cox discovered that rickettsiae could be cultivated successfully in the yolk-sacs of chick embryos

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In 1938, Herald R. Cox discovered that rickettsiae could be cultivated successfully in the yolk-sacs of chick embryos. During World War II, all rickettsial vaccines were produced by this method.

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Source: JSTOR
Credit: Photo: Harold Cox, courtesy of the Rocky Mountain Laboratories, National Institutes of Health (John William Johnson, 1878-1957)