In 1892, the original building of the Wistar Institute was constructed, and today still a part of the Institute’s research facility, was designed by Philadelphia architects George W. and William G. Hewitt, and sits at the corner of 36th and Spruce streets.

The Wistar Institute building, located on the northwest side of Spruce and 36th Street, was constructed in 1894, with 1897 addition, G.W. & W.D. Hewitt architects.

The Institute is listed in the National Register of Historic Places as part of the historic university area, and is also recognized by a historic marker from the Pennsylvania Historical and Museum Commission.